Story wise, I think the Leviathan DLC adds a huge junk that you would want. Personally I didn't play until the reworked ending as well. What bugged me the most was in the end I really only had two options, sacrifice EDI and Joker's happiness, or kill myself. could never bring myself to sacrifice EDI so...
I've yet to see the Illusive Man commit suicide. I think the 2-3 renegade options I always pick--because lets face it, we all wanted to punch that damn reporter--always screw me.
By far I think the standouts for my first play through was my interaction with Garrus and Mordin and the ending with Anderson. He's been my go to bro since Mass Effect 1 other than Wrex and the Citadel can shooting scene was perfect, as well as the conversation prior to the final push. And Mordin, well done character, simply well done. The music for Anderson's final scene will stay with me for a while.
My most disliked squad mates had to be Ashley, Kaiden, Miranda, and Zaeed. I just couldn't bring myself to like them.
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I played mostly paragon with the occasional renegade interrupt like killing udina. Maybe it was because it was an imported character from me1.