JavaScript is required to use Bungie.net

Forums

Edited by Vicex: 12/7/2013 6:51:25 AM
2
How do I get rid of Triton X from my protein sample so that I can crystallise it...without diluting my protein sample too much? I need about 20 mg/mL... and I only can get .06 mg/mL at the moment.
English

Posting in language:

 

Play nice. Take a minute to review our Code of Conduct before submitting your post. Cancel Edit Create Fireteam Post

  • If your protein wont aggregate (or precipitate) with lower amount of detergent, I would say try incubating the concentrated protein in Biobeads to remove some of the concentrated TX-100. The removal of TX-100 can be easily monitored by UV absorption or fluorescence as TX-100 does absorb around 280 nm wavelength. I have not come across the mixing detergent technique, so that sounds interesting as well. If this indeed decreases your micellar size, a good way to check would be using light scattering I suppose! I hope this help! Good luck!

    Posting in language:

     

    Play nice. Take a minute to review our Code of Conduct before submitting your post. Cancel Edit Create Fireteam Post

  • Was expecting a crap answer.... but actually makes sense. I'm impressed.

    Posting in language:

     

    Play nice. Take a minute to review our Code of Conduct before submitting your post. Cancel Edit Create Fireteam Post

You are not allowed to view this content.
;
preload icon
preload icon
preload icon