How do I get rid of Triton X from my protein sample so that I can crystallise it...without diluting my protein sample too much?
I need about 20 mg/mL... and I only can get .06 mg/mL at the moment.
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If your protein wont aggregate (or precipitate) with lower amount of detergent, I would say try incubating the concentrated protein in Biobeads to remove some of the concentrated TX-100. The removal of TX-100 can be easily monitored by UV absorption or fluorescence as TX-100 does absorb around 280 nm wavelength. I have not come across the mixing detergent technique, so that sounds interesting as well. If this indeed decreases your micellar size, a good way to check would be using light scattering I suppose! I hope this help! Good luck!
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Was expecting a crap answer.... but actually makes sense. I'm impressed.